Electrochemical fluidized bed reactors for electroenzymatic syntheses including gaseous phases – Part II: Modeling, scale-up and in-line process monitoring

During the initial phase, the state of research of fluidized bed electrodes and their use in bioelectrochemical reactors has been thoroughly introduced. This project aims to further develop innovative, efficient, and scalable electrochemical fluidized bed reactors for electroenzymatic and possibly electromicrobial synthesis, incorporating gaseous phases. We assess the reactor's efficiency through in-situ hydrogen peroxide generation, which is directly consumed by peroxygenases for oxyfunctionalization reactions of various substrates. The application-oriented design of the fluidized bed electrode emphasizes scalability and practicality, facilitating continuous operation and easy enzyme carrier replacement without disassembly of the reactor. Additionally, we intend to integrate sensitive, rapid mass spectrometry for real-time chemical performance monitoring. Coupled with potentiostat-monitored electrical indicators, this enables improved process understanding and swift testing of new substrates, cofactors and enzymes without the need for laborious assays. Furthermore, we will expand our modeling efforts to refine reactor design and assist other SPP members in identifying, addressing and overcoming process limitations through simulations.

Publications

Tschöpe A, Franzreb M (2021) Influence of non-conducting suspended solids onto the efficiency of electrochemical reactors using fluidized bed electrodes. Chemical Engineering Journal: https://doi.org/10.1016/j.cej.2021.130322

Klaiber M, Tschöpe A, Cu K, Waibel I, Heißler S, Franzreb M, Lahann J (2022) Multifunctional Core–Shell Particle Electrodes for Application in Fluidized Bed Reactors. ACS Applied Engineering Materials: https://doi.org/10.1021/acsaenm.2c00072

Sapotta B, Schwotzer M, Franzreb M (2022) Practical Insights into the Impedance Response of Interdigitated Electrodes: Extraction of Relative Static Permittivity and Electrolytic Conductivity. Electroanalysis: https://doi.org/10.1002/elan.202200102

Greifenstein R, Ballweg T, Hashem T, Gottwald R, Achauer D, Kirschhöfer F, Nusser M, Brenner-Weiß G, Sedghamiz E, Wenzel W, Mittmann E, Rabe K, Niemeyer C, Franzreb M, Wöll C (2022) MOF-Hosted Enzymes for Continuous Flow Catalysis in Aqueous and Organic Solvents. Angewandte Chemie: https://doi.org/10.1002/anie.202117144

Bolat S, Greifenstein R, Franzreb M und Holtmann D (2023) Process intensification using immobilized enzymes. Physical Sciences: https://doi.org/10.1515/psr-2022-0110

Sayoga G, Abt M, Teetz N, Bueschler V, Liese A, Franzreb M, Holtmann D (2023) Quantitative and non-quantitative assessments of enzymatic electrosynthesis: a case study of parameter requirements. ChemElectroChem: e202300226. https://doi.org/10.1002/celc.202300226

Abt M, Franzreb M, Jestädt M, Tschöpe A (2023) Three-phase fluidized bed electrochemical reactor for the scalable generation of hydrogen peroxide at enzyme compatible conditions. Chemical Engineering Journal: https://doi.org/10.1016/j.cej.2023.146465