Next Generation electroenzymatic Cascades for complex molecules (NGeCascades)

The aurachins constitute a large family of isoprenoid quinolone antibiotics isolated from the myxobacteria Stigmatella aurantiaca and S. erecta. Their biosynthesis has been investigated by feeding studies as well as genetic experiments revealing anthranilic acid and acetate as precursors. Due to their structure and biological activity, the aurachins represent a good model system to study the integrated use of organic synthesis, biocatalysis and electroenzymatic ATP supply. Each individual system of this cascade, the electroorganic synthesis of an N-hydroxyquinolone library, the prenylation of these derivatives with a recombinant prenyltransferase to aurachin derivatives as well as an electroenzymatic production of ATP has not been described previously and is in itself of high novelty. Moreover, such a combination is unprecedented for the synthesis of such complex molecules. Therefore, fundamental questions of compatibility and engineering of the cascade are raised and will be investigated by this project in addition to establishing the individual reaction steps.

Project overview