Rational design of 3-D electroenzymatic electrodes comprising novel enzymatic cascade and NADP(H) co-factor regeneration

In this project the rational design of 3-D porous enzymatic electrodes comprising a novel enzymatic cascade with electrochemical nicotinamide adenine dinucleotide phosphate (NAD(P)H) co-factor regeneration is targeted. This will be exemplified for flavin-dependent enzymes such as Baeyer-Villiger monooxygenases in combination with enoate reductases, which both require NAD(P)H. For both enzymes, chiral lactones will be the target compounds, which can also serve as polymer precursors. We aim for a direct NAD(P)H regeneration at the electrode surface, without any further components, except electrons, involved. For the direct co-factor regeneration, the use of carbon/metal composite electrodes is of particular interest; especially with respect to stability issues and the possibility to achieve higher current densities. The selectivity of metal electrodes towards the enzymatically active NAD(P)H form will be tuned by electrode potential, surface modifications, and electrode nano structuring. Rational protein design and directed evolution will be used to adapt the enzymes to conditions required for the electroenzymatic reactor.

Project overview